Journal: Scientific Reports

Article Title: A novel diagnosis strategy for chronic inflammatory bowel diseases using immuno-PET of [ 89 Zr]Zr-IL-23p19 antibody

doi: 10.1038/s41598-025-29881-8

Figure Lengend Snippet: Schematic illustration of the [ 89 Zr]Zr-IL-23p19 antibody preparation and PET/MR imaging workflow for IBD evaluation.

Article Snippet: The anti-mouse IL-23p19 monoclonal antibody (BioXcell, Lebanon, NH, USA) was first purified using a PD-10 desalting column (GE Healthcare, Buckinghamshire, UK) and eluted with saline at a concentration of 3.3 mg/mL.

Techniques: Positron Emission Tomography-Magnetic Resonance Imaging, Imaging

Journal: Scientific Reports

Article Title: A novel diagnosis strategy for chronic inflammatory bowel diseases using immuno-PET of [ 89 Zr]Zr-IL-23p19 antibody

doi: 10.1038/s41598-025-29881-8

Figure Lengend Snippet: Radiolabeling and quality control of the 89 Zr-labeled antibody ([ 89 Zr]Zr-IL-23p19). ( a ) Schematic illustration of the radiolabeling of [ 89 Zr]Zr-IL-23p19. ( b ) Radiolabeling efficiency of [ 89 Zr]Zr-IL-23p19 at 10, 30, and 60 min reaction times. ( c ) Representative radio-TLC chromatogram of the purified [ 89 Zr]Zr-IL-23p19. ( d ) Synthetic scheme of [ 18 F]FSPG, included to provide a complete overview of both radiotracers used in the study.

Article Snippet: The anti-mouse IL-23p19 monoclonal antibody (BioXcell, Lebanon, NH, USA) was first purified using a PD-10 desalting column (GE Healthcare, Buckinghamshire, UK) and eluted with saline at a concentration of 3.3 mg/mL.

Techniques: Radioactivity, Control, Labeling, Purification

Journal: Scientific Reports

Article Title: A novel diagnosis strategy for chronic inflammatory bowel diseases using immuno-PET of [ 89 Zr]Zr-IL-23p19 antibody

doi: 10.1038/s41598-025-29881-8

Figure Lengend Snippet: Successful establishment of DSS-induced colitis in murine IBD models. ( a ) Body weight changes following DSS treatment compared to control mice ( n = 6 per group). ( b ) Macroscopic comparison of colons from control and DSS-induced colitis mouse models. ( c ) Representative H&E-stained colon sections showing histological features of control and DSS-treated groups. Scale bar: 100 μm. ( d ) Immunohistochemical staining for IL-23p19 expression in colonic tissues. Scale bar: 100 μm. ( e ) Quantification of IL-23p19 expression. Data are presented as mean ± standard deviation ( n = 6 per group). ( f ) Cytokine profile analysis comparing DSS-treated and control groups ( n = 6 per group).

Article Snippet: The anti-mouse IL-23p19 monoclonal antibody (BioXcell, Lebanon, NH, USA) was first purified using a PD-10 desalting column (GE Healthcare, Buckinghamshire, UK) and eluted with saline at a concentration of 3.3 mg/mL.

Techniques: Control, Comparison, Staining, Immunohistochemical staining, Expressing, Standard Deviation

Journal: Scientific Reports

Article Title: A novel diagnosis strategy for chronic inflammatory bowel diseases using immuno-PET of [ 89 Zr]Zr-IL-23p19 antibody

doi: 10.1038/s41598-025-29881-8

Figure Lengend Snippet: PET/MR imaging and quantitative analysis of DSS-induced mouse models. ( a ) Representative axial and coronal PET/MR fusion images and corresponding T1-weighted MR images of control (left), [ 89 Zr]Zr-IL-23p19–injected (center), and [ 18 F]FSPG–injected (right) mice. SUV mean of [ 89 Zr]Zr-IL-23p19 ( b ) and [ 18 F]FSPG ( c ). Lesion-to-muscle uptake ratio of [ 89 Zr]Zr-IL-23p19 ( d ) and [ 18 F]FSPG ( e ). Data are presented as mean ± SD ( n = 6 per group).

Article Snippet: The anti-mouse IL-23p19 monoclonal antibody (BioXcell, Lebanon, NH, USA) was first purified using a PD-10 desalting column (GE Healthcare, Buckinghamshire, UK) and eluted with saline at a concentration of 3.3 mg/mL.

Techniques: Positron Emission Tomography-Magnetic Resonance Imaging, Imaging, Control, Injection

Journal: Scientific Reports

Article Title: A novel diagnosis strategy for chronic inflammatory bowel diseases using immuno-PET of [ 89 Zr]Zr-IL-23p19 antibody

doi: 10.1038/s41598-025-29881-8

Figure Lengend Snippet: Correlation analysis between PET imaging signals and DAI in DSS-induced models. ( a , b ) [ 89 Zr]Zr-IL-23p19 uptake in the intestine showed a strong positive correlation with DAI scores on day 10 (R 2 = 0.7302) and day 14 (R 2 = 0.9122) post-DSS treatment. ( c , d ) [ 18 F]FSPG uptake demonstrated no significant correlation with DAI scores on day 10 (R 2 = 0.0106) or day 14 (R 2 = 0.4595), indicating limited ability to reflect disease severity.

Article Snippet: The anti-mouse IL-23p19 monoclonal antibody (BioXcell, Lebanon, NH, USA) was first purified using a PD-10 desalting column (GE Healthcare, Buckinghamshire, UK) and eluted with saline at a concentration of 3.3 mg/mL.

Techniques: Imaging